To West Preston

Trams commence in Park Street near Fitzroy Street (St Kilda) and run via Patterson Street, Danks Street, Mills Street, Albert Road, Clarendon Street, Spencer Street, Collins Street (City), Macarthur Street, St Vincents Plaza, Brunswick Street, St Georges Road, Miller Street and Gilbert Road to terminate near Regent Street (Preston).

To South Melbourne/St Kilda Beach

Trams commence in Gilbert Road near Regent Street (Preston) and run via Gilbert Road, Miller Street, St Georges Road, Brunswick Street, St Vincents Plaza, Macarthur Street, Collins Street (City), Spencer Street, Clarendon Street, Albert Road, Mills Street, Danks Street, Patterson Street and Park Street to Fitzroy Street (St Kilda).