To Box Hill Central

Trams commence at Waterfront Place (Port Melbourne) and run via the Light Rail, Clarendon Street, Spencer Street, Collins Street (City), Macarthur Street, St Vincents Plaza, Victoria Parade, Victoria Street, Barkers Road, High Street, Cotham Road and Whitehorse Road to the terminus near Market Street (Box Hill).

To Port Melbourne

Trams commence in Whitehorse Road near Market Street (Box Hill) and run via Whitehorse Road, Cotham Road, High Street, Barkers Road, Victoria Street, Victoria Parade, St Vincents Plaza, Macarthur Street, Collins Street (City), Spencer Street, Clarendon Street and the Light Rail to Waterfront Place (Port Melbourne).