To Bundoora/RMIT

Trams commence in Docklands Drive near Waterfront City (Docklands) and run via Harbour Esplanade, Latrobe Street, Spencer Street, Bourke Street (City), Spring Street, Nicholson Street, Gertrude Street, Smith Street, Queens Parade, High Street and Plenty Road to terminate near McKimmies Road (Bundoora).

To Waterfront City/Docklands

Trams commence in Plenty Road near McKimmies Road (Bundoora) and run via Plenty Road, High Street, Queens Parade, Smith Street, Gertrude Street, Nicholson Street, Spring Street, Bourke Street (City), Spencer Street, Latrobe Street, Harbour Esplanade and Docklands Drive to Waterfront City (Docklands).