To Garden City

Departs from the La Trobe University Bus terminal near the corner of Plenty Road and Kingsbury Drive then travels via Science Drive, Ring Road, Waterdale Road, Dougharty Road, Oriel Road, Bell Street, Oriel Road, Darebin Road, Victoria Road, Westgarth Street, High Street, Queens Parade, Rushall Crescent, Park Street, Bennett Street, Holden Street, Brunswick Road, Amess Street, Park Street, Rathdowne Street, Exhibition Street and Lonsdale Street to the city terminus near Melbourne Central. From City bus continues via Lonsdale Street, Queen Street, Flinders Street, Queens Bridge Street, City Road, Crockford Street, Bay Street, Graham Street, Williamstown Road, Beacon Road, Howe Parade to the terminus at the corner of Centre Avenue and Howe Parade. EXTENDED SERVICE: Route 250/251/253 is a premium service, featuring frequent service and extended operating hours. RELATED ROUTES: 251 & 253.

To La Trobe University

Departs from the terminus at the corner of Centre Avenue and Howe Parade and travels via Howe Parade, Beacon Road, Williamstown Road, Graham Street, Bay Street, Crockford Street, City Road, Queens Bridge Street, Flinders Street, Queen Street and Lonsdale Street to the City terminus near Melbourne Central. From City terminus bus continues via Lonsdale Street, Exhibition Street, Rathdowne Street, Park Street, Amess Street, Brunswick Road, Holden Street, Bennett Street, Park Street, Rushall Crescent, Queens Parade, High Street, Westgarth Street, Victoria Road, Darebin Road, Oriel Road, Bell Street, Oriel Road, Dougharty Road, Waterdale Road, Ring Road, Science Drive to the La Trobe University Bus terminal near the corner of Plenty Road and Kingsbury Drive. EXTENDED SERVICE: Route 250/251/253 is a premium service, featuring frequent service and extended operating hours. RELATED ROUTES: 251 & 253.