To East Brunswick

Trams commence in Acland Street near Barkly Street (St Kilda) and run via Acland Street, The Esplanade, Fitzroy Street, South Melbourne Light Rail (Canterbury Road, Ferrars Street and Whiteman Street), Clarendon Street, Spencer Street, Bourke Street (City), Spring Street and Nicholson Street to terminate near Bell Street (Coburg).

To St Kilda Beach

Trams commence at Nicholson Street near Bell Street (Coburg) and run via Nicholson Street, Spring Street, Bourke Street (City), Spencer Street, Clarendon Street, South Melbourne Light Rail (Whiteman Street, Ferrars Street and Canterbury Road), Fitzroy Street, The Esplanade and Acland Street near Barkly Street (St Kilda).